Noninvasive prenatal diagnosis of fetal aneuploidy by circulating fetal nucleated red blood cells and extravillous trophoblasts using silicon-based nanostructured microfluidics

Huang, Chung-Er; Ma, Gwo-Chin; Jou, Hei-Jen; Lin, Wen-Hsiang; Lee, Dong-Jay; Lin, Yi-Shing; Ginsberg, Norman A.; Chen, Hsin-Fu; Chang, Frank Mau-Chung; Chen, Ming

doi:10.1186/s13039-017-0343-3

Molecular Cytogenetics

Table 3 Summary of the STR results for the captured fetal cells (fnRBC and/or EVT) from the 5 pregnant women. For each case, at least 4 informative STR loci are feasible to distinguish the fetal cells from the maternal cells (the non-maternal alleles are marked in bold)

From: Noninvasive prenatal diagnosis of fetal aneuploidy by circulating fetal nucleated red blood cells and extravillous trophoblasts using silicon-based nanostructured microfluidics

Locus	Case 1 (Trisomy 13)		Case 2 (Trisomy 18)				Case 3 (Trisomy 21)			Case 4 (Disomy: 46,XY)		Case 5 (Disomy: 46,XX)
Locus	Maternal leukocyte	fnRBC	Maternal leukocyte	fnRBC	EVT	Abortus tissue	Maternal leukocyte	fnRBC	EVT	Maternal leukocyte	fnRBC	Maternal leukocyte	fnRBC
D3S1358	133, 137	129, 137	129, 137	129, 137	129, 137	129, 137	129, 137	129, 137	129, 137	129, 133	133, 137	129, 133	129, 133
TH01	171, 179	171, 183	167, 171	167, 179	167, 179	167, 179	171, 179	171, 179	171, 179	179	179	179	167, 179
D13S317	182, 190	182, 198	197	181, 197	181, 197	181, 197	181, 197	181, 197	181, 197	182, 194	186, 194	182, 186	182, 186
D8S1179	222, 234	218, 222	238	218, 238	218, 238	218, 238	230, 234	230	230	218, 230	218, 230	231, 239	231
D7S820	234, 238	230, 238	234	234, 238	234, 238	234, 238	226, 238	226, 238	226, 238	242, 246	234, 242	231, 243	231, 235
TPOX	272, 276	272	272	272, 284	272, 284	272, 284	272	272, 276	272, 276	272, 284	272	272, 284	284
D16S539	285	285	285, 297	289, 297	289, 297	289, 297	284	284	284	289, 297	293, 297	285, 301	297, 301
D18S51	307, 311	311	303, 315	303, 315	303, 315	303, 315	307, 319	307, 315	307, 315	307	307	315, 337	303, 315
CSF1PO	315	315, 320	344	340, 344	340, 344	340, 344	336, 344	336, 344	336, 344	344	332, 344	341	341, 349
Penta D	404, 419	404, 419	405, 414	405, 414	405, 414	405, 414	400, 424	424, 433	424, 433	419, 433	419, 433	404, 414	404, 433
Penta E	418, 451	418	429	424, 429	424, 429	424, 429	414, 419	419, 450	419, 450	445, 450	424, 450	414, 435	435, 451
AMEL	105	105	105	105	105	105	105	105	105	105	105, 111	105	105

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ISSN: 1755-8166

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