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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis

Fig. 1

The scheme of Y14–Myc mRNA constructing (a,b,c,d) and Y14 genomic construction (c’). a, Y14 (506 bp) cloned in pcDNA 3.1 Myc–His; b, Y14 + Myc tag (542 bp) cloned in PTZ19R; c, PCR (639 bp) matrix for in vitro transcription; d, Y14–Myc mRNA (609 bp) transcribed in vitro. Yellow, T7 promoter; orange, Y14 coding sequence; light green, Myc epitope; red circles, M13 primers; brown, 5' cap analog; dark green, parts of the vector not significant in this case

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Molecular Cytogenetics

ISSN: 1755-8166