Table 3 PCR primers used in monomer amplification in P.pratensis
From: Isolation and characterization of chromosomal markers in Poa pratensis
Monomers | Sequence (5-- > 3') | Annealing temperature (°C) |
|---|---|---|
Clone 6-F (PpCR-1)R | ACCGTGAACTCTGCGTCG | 53 |
Clone 6-R (PpCR-1)F | TGGACTACCGACGCAGAGTTCAC | |
Clone 1-F (PpTR-1)R | AAGTTCTCAAGGTTTTACCTTCACC | 55 |
Clone 1-R (PpTR-1)F | GGACTACCGACGCAGAGTTCA | |
Clone 23-F (PpTR-2)R | AATCACGTCTTGTGACCGAG | 55 |
Clone 23-R (PpTR-2)F | GGCTCGGTCACAAGACG | |
Clone 94-F (PpTR-3)R | AAATTGATGCTTGACTAGTTGGTGA | 53 |
Clone 94-R (PpTR-3)F | CAGCAAATACACTACTCCAGC |