Fig. 1
FISH image of an embryonic blastomere from the untransferred embryo no. 10 (Table 3) belonging to couple A with karyotype 46,X,t(Y;4)(q12;p15.32). The FISH signal pattern is of an unbalanced male embryo consistent with 2 × CEP 4 (SA), 3 × Tel 4p(SG), 1 × CEP X(SO) in the 1st round and 1 × CEP Y (der Y) (SA), 1 × Tel Xq/Yq (SO) in the second round of hybridisation. The expected FISH signals pattern for an embryo to be a balanced male would be 2 × CEP 4 (SA), 2 × Tel 4p(SG), 1 × CEP X(SO) in the 1st round of hybridisation and 1 × CEP Y(der Y)(SA), 2 × Tel Xq/Yq (SO) in the 2nd round. Meiotic segregation analysis revealed that chromosomes der Y and 4 was the contribution from the male gamete