Fig. 3

Cellular morphologies and karyotypes of melanoma WM-115 (a, b) and a corresponding metastasis WM-266-4 (c, d). The comparisons show that the primary cancer WM-115 and the corresponding metastasis WM-266-4 have similar, but distinct cell morphologies (a, c) and karyotypes (b, d). Both primary cancer and metastasis have hyper-triploid karyotypes with similar numbers of chromosomes and aneusomies and both lack intact chromosome 9. They also differ from each other in the total numbers of chromosomes and in the structures of some marker chromosomes (see Tables 1 and 2). The absence of normal chromosomes 6 from the presumed primary and the presence of "primary specific" marker chromosomes 6 indicate that both the presumed primary and the metastasis derived from an unknown primary with normal chromosomes 6. The cells were propagated and karyotyped as described for Fig. 2