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Fig. 2 | Molecular Cytogenetics

Fig. 2

From: Effect of SP600125 on the mitotic spindle in HeLa Cells, leading to mitotic arrest, endoreduplication and apoptosis

Fig. 2

a Histone 3 Phosphorylation was analyzed using immunofluorescent staining in HeLa cells treated with 20 μM SP600125 for the indicated times. Cells were fixed, permeabilized, and stained. Tubulin tagged with IgG anti-tubulin, H3 phosphorylated was stained with Rabbit Anti-Histone H3 (phospho S10) antibody (ab47297). Primary antibodies was detected using an anti-mouse and anti- rabbit secondary antibodies conjugated with Alexa Fluorescent (Green for tubulin and Red for H3P). Cells were analyzed using fluorescence microscopy (×400). Hoechst 33258 was used for nuclear staining. b Endoreduplication detected using Immunofluorescence in HeLa cells treated with 20 μM SP600125 for 48H. Tubulin tagged with IgG anti-tubulin. Primary antibody was detected using an anti- mouse secondary antibody conjugated with Alexa Fluorescent (Green for tubulin)

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Molecular Cytogenetics

ISSN: 1755-8166