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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: Effect of SP600125 on the mitotic spindle in HeLa Cells, leading to mitotic arrest, endoreduplication and apoptosis

Fig. 1

a HeLa cells were plated at 5 × 104 cells/ml and incubated for 24 h. Cells were treated with the indicated concentrations of SP600125 and the cell viability was measured using a metabolic-dye-based MTT assay. Each point represents the mean ± SD of three independent experiments. Significance was determined using Student’s test (*P < 0.05 vs. vehicle control). a’ HeLa cells were plated at 5 × 104 cells/ml and incubated for 48 h. Cells were treated with the indicated concentrations of SP600125 and the cell viability was measured using a metabolic-dye-based MTT assay. Each point represents the mean ± SD of three independent experiments. Significance was determined using Student’s test (*P < 0.05 vs. vehicle control). a”: HeLa cells were plated at 5 × 104 cells/ml and incubated for 72 h. Cells were treated with the indicated concentrations of SP600125 and the cell viability was measured using a metabolic-dye-based MTT assay. Each point represents the mean ± SD of three independent experiments. Significance was determined using Student’s test (*P < 0.05 vs. vehicle control). b Cells were incubated with the indicated concentrations of SP600125. After a 48 h-incubation, cells were sampled and examined under light microscopy. Magnification × 200. c After HeLa cells were treated with SP600125 for the indicated time, expression of c-Jun and phosphorylated c-Jun was detected using Western blot analysis

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