Skip to main content

Table 2 Comparison between cell soring by immunomagnetic beads and Target FISH

From: Target fluorescence in-situ hybridization (Target FISH) for plasma cell enrichment in myeloma

Cell sorting by immunomagnetic beads Target FISH
Procedure Time (hours) Reagent Cost (USD) Procedure Time (hours) Reagent Cost (USD)
Day 1 Plasma cell sorting by MACS whole blood CD138 μ-beads 1.5 60 Day 1 Plasma cell enrichment by Ficoll 1.5 12
Cytospin slides (10 slides per sample) 0.5 29
Cytospin slides (10 slides per sample) 0.5 29 MGG staining 0.5 9
BioView scan and review 2.0 0
Day 2 Lysis of red blood cells on slide 0.5 2 Day 2 De-staining of MGG 1.5 2
FISH hybridization 3.0 236 FISH hybridization 3.0 236
Probe hybridization Overnight 0 Probe hybridization Overnight 0
Day 3 Post-hybridization 1.0 13 Day 3 Post-hybridization 1.0 13
Manual slide examination 2.0 0 BioView slide scan 3–4 0
Review results, data analysis and reporting 2.0 0 Review results, data analysis and reporting 2.0 0
Total 10.5 340 Total 15–16 301