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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: The major horse satellite DNA family is associated with centromere competence

Fig. 1

Identification and analysis of the CENP-A bound horse satellite. a: In the left panel, the enrichment of the 37cen, 2P1 and EC37 satellites was measured as ratio between normalized read counts in immunoprecipitated and in input DNA. The same calculation was performed for the ERE1 retrotransposon sequence. The right panel shows the 221 bp consensus sequence of the CENP-A bound 37cen satellite. b: Slot-blot analysis. Left panel: hybridization of P32 labelled probes (37cen, 2PI and ERE-1) with DNA purified from chromatin immunoprecipitated with CENP-a (top) and from non immunoprecipitated chromatin (bottom). Right panel: densitometric analysis of slot-blot hybridizations

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