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Table 1 List of antibodies targeting components of active centromere. Antibodies were first tested on non-fixative treated cells to determine optimal concentration or dilution for immunocytochemistry and then tested on cells stored in methanol-acetic acid fixative

From: Active centromere and chromosome identification in fixed cell lines

 

Antibody

Species

Concentration or dilution

Cells stored in methanol-acetic acid

1

BubR1 [EPR12259(2)] ab183496 (Abcam)

Rabbit IgG monoclonal

0.527 μg/ml

No

2

CENP-A (Clone: 3–19)

Mouse IgG monoclonal

1 : 500

No

3

CENP-A phospho Ser18 (Active Motif)

Rabbit IgG polyclonal

1 : 400

No

4

CENP-C (Serum from rabbit 554; gift from William Earnshaw)

Rabbit polyclonal

1 : 1000

Yes

5

CENP-C [EPR15939] ab193666 (Abcam)

Rabbit IgG monoclonal

0.677 μg/ml

Yes

6

CENP-E [EPR4542(2)] ab133583 (Abcam)

Rabbit IgG monoclonal

0.275 μg/ml

Not homogeneous across all chromosomes

7

HEC1 [9G3] ab3613 (Abcam)

Mouse IgG monoclonal

0.5 μg/ml

No