Fig. 4
From: Active centromere and chromosome identification in fixed cell lines
CENP-IF-cenFISH-mFISH performed on cancer cell lines T-47D and SN12C with neocentric and dicentric chromosome respectively. a Upper panel: immunofluorescence images for T-47D showing α-satellite (green), CENP-C (red) and DAPI (blue); boxed in red is the neocentric chromosome and insets are enlarged images of the neocentric chromosome. Lower panel: mFISH colour profile of the neocentric chromosome indicating it is a rearranged chromosome containing segments from chromosomes 3 and 5. b Upper panel: immunofluorescence images for SN12C showing α-satellite (green), CENP-C (red) and DAPI (blue); boxed in red is the dicentric chromosome and insets are enlarged images of the dicentric chromosome. Lower panel: mFISH colour profile of the dicentric chromosome indicating it is a rearranged chromosome from chromosomes 21 and 10