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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: Why it is crucial to analyze non clonal chromosome aberrations or NCCAs?

Fig. 1

Examples of NCCAs: a DMF image (reversed DAPI image) detected from mouse cell culture. Left portion shows the de-condensed chromosomes are tangling together, while some normal condensed chromosomes are nearby. b SKY image of a chaotic genome detected from a Dox treated mouse cell. Each normal chromosome should have one unique color. However, for these massively re-organized chromosomes, there are multiple colors detected from each single chromosome, indicating the multiple events of chromosomal shattering and stitching. Note that there are many extremely long chromosomes. c An image of a giant nucleus (DAPI image) detected from HT-29 cells cultured in situ. Typical normal-sized nuclei are surrounding the giant nucleus. d An image of a cluster of cells derived from one giant nucleus. Since many of these cells are stochastically generated and display different amounts of DNA, these cells represent NCCAs when they enter into metaphase. Live imaging shows that there are continuous division /fusion events for unstable cancer cells, suggesting a new means of generating fuzzy inheritance

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