Table 1 Primary and secondary antibodies used for immunofluorescence
Antibodies | Raised/type | Source | Catalog no. |
|---|---|---|---|
Anti-5-methylcytosine (5meC) (Clone 10G4) | Mouse monoclonal | Zymo | A3001 |
Anti-H3K4me2 | Rabbit polyclonal | Upstate (Millipore) | 07–030 |
Anti-H3K27me3 | Rabbit polyclonal | Upstate (Millipore) | 07–449 |
Anti-H3K9me2 | Rabbit polyclonal | Upstate (Millipore) | 07–441 |
Anti-H3K4me3 | Mouse monoclonal | Abcam | ab–1012 |
Anti-Cy3 anti-mouse | Donkey polyclonal | Jackson Immunoresearch Laboratories | 715–165–151 |
Anti-FITC anti-rabbit | Donkey polyclonal | 711–095–152 |