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Fig. 4 | Molecular Cytogenetics

Fig. 4

From: Immunofluorescent staining reveals hypermethylation of microchromosomes in the central bearded dragon, Pogona vitticeps

Fig. 4

Distribution of repressive epigenetic marks across Pogona vitticeps metaphase chromosomes. Distribution of H3K27me3: (a) DAPI stained chromosomes, (b) merged image with H3K27me3 staining in green and DAPI in blue, (c) Representative line scans of staining on a macrochromosome (red) and microchromosome (yellow). The blue curves correspond to the DAPI staining along the length of the chromosomes. The green curves show the distribution of H3K27me3. d Karyotype of chromosomes depicted in image b. Distribution of H3K4me3: (e) DAPI stained chromosomes, (f) merged image showing H3K9me2 staining in green and DAPI staining in blue. g Representative line scans of staining on a macrochromosome (red) and microchromosome (yellow). h Karyotype of chromosomes depicted in image g. Scalebars represent 10 μm

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