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Fig. 8 | Molecular Cytogenetics

Fig. 8

From: Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells

Fig. 8

Non-correlations between SV40 T-antigen and the cells of the immortal clones F1 and F4. To answer the question whether clonal cancer karyotypes are sufficient to generate and maintain neoplastic clones or are also dependent on T-antigen, we analyzed the immortal neoplastic clones F1 and F4 for the presence of viral T- antigen. For this purpose F1 and F4 cell cultures were reacted with mouse anti-T-antigen antibodies-linked to a green fluorescent dye and counter-stained with the blue fluorescent DNA dye, ‘DAPI,’ to detect nuclear DNA irrespective of T-antigen (Methods). a shows that about 30 % of the cells of the F1 line were T-antigen negative, while the remaining 70 % were heterogeneous for T-antigen expression ranging from very low to relatively high levels. b shows that about 50 % of the cells of F4 were T-antigen negative, whereas the rest of the cells were heterogeneous ranging from very low to relatively high T-antigen levels, similar to the F1 culture. We conclude that T-antigen is not necessary to maintain neoplastic transformation of F1 and F4 lines

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