Fig. 7
From: Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells
Comparison of the karyotype array of the F4 line with that of a puromycin-resistant derivative. To determine whether resistance to puromycin of the F4 line was acquired by karyotypic variation, as was found in Fig. 6, the karyotype array of a puromycin-resistant F4 variant was compared to that of the parental F4 line. The array of the drug-resistant F4 shows a hypo-tetraploid karyotype consisting of 80 chromosomes that were 55–100 % clonal. Accordingly, these chromosomes formed a quasi-clonal array, which is distinct from, but visibly related to from the parental F4 line, shown in Fig. 5b. Specifically, the puromycin-resistant F4 variant differs from the parental line in the copy numbers of 15 of their 34-shared chromosomes (marked yellow in Fig. 7), the gain of two resistance-specific marker chromosomes and the loss of four parental marker chromosomes. We conclude that the F4 line acquired resistance to puromycin by karyotypic variation, as was the case with the puromycin-resistant variant of the F1 clone described in Fig. 6