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Fig. 6 | Molecular Cytogenetics

Fig. 6

From: Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells

Fig. 6

Comparison of the karyotype array of the F1 line with that of a puromycin-resistant derivative. To determine whether acquisition of resistance to puromycin was based on karyotypic variation, the karyotype array of a puromycin-resistant variant of F1was compared to that of the parental F1 clone. The array of the drug-resistant F1 shows a hyper-tetraploid karyotype consisting of 105 chromosomes that were 45–100 % clonal. Accordingly these chromosomes formed a quasi-clonal, resistant F1-specific array, which is distinct from, but visibly related to that of the parental F1 line, shown in Fig. 5a. Quantitative comparison of the chromosome copy numbers of the two F1 variants shown in the table of Fig. 6 revealed obvious similarities: 14 F1-chromsomes were exactly duplicated in the resistant variant and six others were increased approximately two-fold. It follows that the puromycin-resistant variant of F1 arose from the parental F1 line by an approximate tetraploidization; similar to how the above described near tetraploid F4 arose from the near diploid F1. This event was also associated with the acquisition of 29 new resistance-specific clonal marker chromosomes, and with the loss of three parental clonal marker chromosomes

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