Fig. 3

Cell morphologies of the immortal cell lines F1 and F4 derived from SV40-infected human mesothelial cells. The karyotypic cancer theory predicts that neoplastic karyotypes encode the individual phenotypes of SV40-transformed neoplastic clones. To test this prediction we have compared at 120X magnifications cultures of the immortal neoplastic cell lines F1 and F4, which arose from SV40-infected human mesothelial cells. It can be seen in the micrographs shown in (a) that the F1 line formed a dense monolayer of cells and in (b) that the F4 line formed a multilayer of cells. The micrographs also show that both lines consisted of round to oval polymorphic cells, and that the F4-cells were on average larger than the F1 cells. This result revealed phenotypic cellular similarity but sociological dissimilarity of the two cell lines