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Fig. 14 | Molecular Cytogenetics

Fig. 14

From: Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells

Fig. 14

Non-correlations between the cells of two SV40-induced neoplastic clones of rat lung cells and SV40 T-antigen. To test the prediction of the karyotypic theory that the clonal karyotypes of SV40-induced neoplastic clones are sufficient to generate and maintain neoplastic clones, independent of the viral T-antigen, we analyzed the cells of the SV40-induced neoplastic rat clones F3 and F100 (described in Figs. 10 and 12) for the presence of viral T-antigen with green-labeled antibodies, as described above for Fig. 8. a shows that about 30 % of the F3 cells were T-antigen negative, while the remaining 70 % were heterogeneous, expressing T-antigen between very low to relatively high levels. b shows that all cells of the neoplastic rat clone F100 were T-antigen negative under the conditions of our test (Methods). We concluded from the absence of detectable T-antigen in F100 and the absence or heterogeneous presence of T-antigen in F3 cells that T-antigen is not necessary to maintain neoplastic transformation of SV40-induced neoplastic rat clones, as predicted by the karyotypic theory

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