Fig. 13

Karyotype arrays of six morphologically distinct focal colonies derived from SV40-infected rat lung cells: third pair of three. To test the theory that individual clonal karyotypes encode individual phenotypes, we analyzed and compared the arrays of a third pair of six individually distinct rat focal colonies. a shows that F10 has a near-diploid karyotype with an average number of 41 chromosomes that were between 70 and 100 % clonal. Accordingly they formed a quasi-clonal F10-specific array, which is different from those of the F8, F33, F3 and F100 colonies. The F10 clone also includes a minor (15 %) tetraploid variant, similar to that found in clone F3 described above (Fig. 12a). b shows that clone FC1 has a near triploid karyotype with 64 chromosomes that were between 55 and 100 % clonal. Accordingly they formed a quasi-clonal FC1-specific array, which differs from those of all five sister colonies described above and in Figs. 11 and 12. Thus the individual karyotypes of the six phenotypically distinct rat clones support the theory that individual karyotypes encode the individual phenotypes of neoplastic clones