Skip to main content

Table 1 Frequency of EGFR gene amplification in esophago-gastric cancer

From: Epidermal Growth Factor (EGFR) copy number aberrations in esophageal and gastro-esophageal junctional carcinoma

Histology

Ethnicity

Technique

Amplification classification

Amplification (%)

Amplification impact on prognosis

Reference

GC

European

Southern Blot

Not documented

2/30 (6.7 %)

Not assessed

Lemoine 1991 [23]

GC

Chinese

FISH

≥ 15 EGFR copies in ≥10 % tumour cells OR ≥40 % cells with ≥4 EGFR copies OR EGFR/CERP7 ratio ~ 1 but cluster of ≥4EGFR copies in ≥10 % cells OR EGFR/CERP7 ratio ≥2 and cluster of ≥4EGFR copies in ≥10 % cells

20/69 (29 %)

Not assessed

YK 2011 [24]

GC

European

FISH

EGFR/ CEP 7 ratio ≥ 2.0

4/82 (4.88 %)

Poorer survival of EGFR amplified cases in multivariate analysis (HR 4.82, 95 % CI 1.32-17.7, p = 0.0176)

Kandel 2014 [25]

ESCC

Japanese

Southern Blot

EGFR/ CEP 7 ratio ≥ 2.0

9/42 (21.4 %)

Not assessed

Itakura 1994 [26]

ESCC

Thai

FISH

Low level: ratio 1.3-2.0, High level: ratio >2.0

8/15 (49 %)

No significant difference in survival in EGFR amplified cases

Sunpaweravong 2005 [27]

ESCC

Japanese

FISH/CGH

FISH: EGFR/CEP 7 ratio ≥ 2.0; CGH: >4 copies of EGFR gene

16/244 (7 %)

No significant difference in survival in EGFR amplified cases

Kato 2013 [28]

ESCC

Japanese

FISH

Low level: 3-10 EGFR signals/cell; High level: cluster of EGFR signals/>10 signals per cell

15/83 (18.1 %)

No significant difference in survival in EGFR amplified cases

Hanawa 2006 [29]

EA and ESCC

European

CISH

CISH + ve: >50 % cells with either tight EGFR clusters or > 4 EGFR copies per cell

2/16 (12.5 %)

Not assessed

Janmaat 2006 [30]

EA

European

FISH

Ratio ≥ 2.0 or presence of tight EGFR gene clusters

7/112 (6.25 %)

Poorer survival of EGFR amplified cases in multivariate analysis (p = 0.0004)

Marx 2010 [31]

EA

N. American NOS

Southern Blot

Ratio ≥ 2.0

7/87 (8.0 %)

Not assessed

Miller 2003 [32]

  1. Abbreviations: CGH comparative genomic hybridisation, CISH chromogentic in situ hybridisation, FISH fluorescence in-situ hybridisation, GC gastric cancer, EA esophageal adenocarcinoma, ESCC esophageal squamous cell carcinoma