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Fig. 3 | Molecular Cytogenetics

Fig. 3

From: Reversing chromatin accessibility differences that distinguish homologous mitotic metaphase chromosomes

Fig. 3

Quantification of inter-homolog fluorescence intensities following chromosome decondensation with ICRF-193. a-f FISH with single copy probes targeting six distinct genomic regions within chromosomes 1q43 (RGS7), 9q34.3 (CACNA1B), 15q13.1 (HERC2), 17p12 (ADORA2B, PMP22:IVS3), and 22q13.33 (ACR) are indicated. For untreated chromosomes (left column, panels a-f, respectively), probe signal is bright on one homolog and appears dim or not visible on corresponding target (*). For chromosomes treated with ICRF-193, (middle column, panels a-f, respectively) probe signal is bright on both homologs. Probes detecting DA exhibited larger differences in inter-homolog DNA probe fluorescence (red box plots in right column: median intensity ratios: from 0.53 to 1, n = 125 cells). ICRF-193-treated chromosomes exhibited smaller differences in DNA probe fluorescence (black box plots in right column: median intensity ratios from 0.08-0.27, n = 121 cells) (p < 0.05; two tail t-test), suggesting that both chromosomal homologs were equally accessible, except at the HERC2 locus, where DA was not completely reversed. In instances where the median is coincident with the upper quartile, it is emphasized by a thick line to show distinction with median in corresponding category. The notation ‘der 17’ refers to a derivative chromosome 17 homolog resulting from a translocation between chromosome Y and 17

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