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Fig. 2 | Molecular Cytogenetics

Fig. 2

From: Construction of BAC contig maps of homoeologous chromosomes A12 and D12 of Gossypium hirsutum L. acc. TM-1

Fig. 2

Identification of screening BAC library with NAU2251 and the loacaction of postive BAC clone with BAC-FISH. a Identification of the BAC clone z84A22 (lane 3) containing the polymorphic locus of NAU2251-165, and 259 L20 (lane 4) containing the polymorphic locus of NAU2251-155 between TM-1 and Hai7124 by SSR marker NAU2251. Lanes 1–4 were Hai7124, TM-1, z84A22, 259 L20 respectively. b FISH image showed that the signals of the polymorphic allele BAC 259 L20 (green signals, arrows) and the signals of polymorphic allele BAC z84A22 (red signals, arrows) were located on A12 and D12 homoeologous chromosomes. M: Marker, H: Hai7124, T: TM-1,1: Z84A22,2: 259 L20

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Molecular Cytogenetics

ISSN: 1755-8166