Table 1 Comparison of workflows using NGS and aCGH for 24-chromosome copy number analysis
aCGH | NGS | ||
|---|---|---|---|
1 | Embryo biopsy | NA | Embryo biopsy |
2 | Sample collection | Reference gDNA (+10 min) | Sample collection |
3 | WGA (3 hrs) | WGA (3 hrs) | WGA (3 hrs) |
4 | Labeling of amplified DNA (1 hrs) | Pooling and labeling of amplified DNA (1 hrs) | Qualification and dsDNA input dilution (20 min) |
5 | Preparation of labeled DNA (1 hrs) | Preparation of labeled DNA (1 hrs) | Tagmentation (20 min) |
6 | Hybridization to array (2 hrs) | PCR amplification (50 min) | |
7 | Washing and Scanning (20 min) | PCR clean-up (30 min) | |
8 | Cytogenomics analysis (40 min) | Library normalization (30 min) | |
9 | NA | Library pooling and loading (10 min) | |
10 | N/A | Sequencing (6 hrs) | |
11 | N/A | Bioinformatics analysis (2 hrs) | |
Total hrs | ~8 hrs | ~14 hrs | |