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Table 1 Comparison of workflows using NGS and aCGH for 24-chromosome copy number analysis

From: Application of next-generation sequencing for 24-chromosome aneuploidy screening of human preimplantation embryos

  aCGH NGS
1 Embryo biopsy NA Embryo biopsy
2 Sample collection Reference gDNA (+10 min) Sample collection
3 WGA (3 hrs) WGA (3 hrs) WGA (3 hrs)
4 Labeling of amplified DNA (1 hrs) Pooling and labeling of amplified DNA (1 hrs) Qualification and dsDNA input dilution (20 min)
5 Preparation of labeled DNA (1 hrs) Preparation of labeled DNA (1 hrs) Tagmentation (20 min)
6 Hybridization to array (2 hrs) PCR amplification (50 min)
7 Washing and Scanning (20 min) PCR clean-up (30 min)
8 Cytogenomics analysis (40 min) Library normalization (30 min)
9 NA Library pooling and loading (10 min)
10 N/A Sequencing (6 hrs)
11 N/A Bioinformatics analysis (2 hrs)
Total hrs ~8 hrs ~14 hrs
  1. NGS: next generation sequencing
  2. aCGH: array comparative genomic hybridization
  3. WGA: whole-genome amplification
  4. NA: not applicable