mRNA and protein expression of OFD1 and TRAPPC2 in primary culture of human chorionic villi. ET 15, 17, 18 and 20 indicate control samples from the cell cultures of elective terminations (n = 4). 09-3A and B indicate miscarriages from female 09–1. mRNA (i) and protein (ii) values for (A) OFD1 and (B) TRAPPC2 expression were normalized to the corresponding ß-actin mRNA levels. The results derived from at least three repeats of each sample and the mean level of each sample is represented in the scatter graph. The difference between the four control samples (as a group) and the two CNV carrier miscarriages, as two groups, has been evaluated by Student t-test (*, p < 0.05).