FISH hybridization after immunodetection of the synaptonemal complex with anti-medaka SYCP3 antibody. Chromosome painting with specific probes for B chromosome in Characidium gomesi
(a, b, c) and histone H1 probe hybridization in Astyanax paranae
(d, e, f). In c and f, FISH hybridization signs are visualized on the specific bivalent chromosomes. Bar = 10 μm.