Figure 3
Dual-FISH with positive BAC clones (green) and chromosome specific BAC clones (red) as probe while chromosomes were counterstained with DAPI. Bar = 5um. A: BAC clone 216B15 tagged by SSR marker NAU4891 (green) and chromosome D501 specific BAC clone tagged by SSR marker BNL3902 (red), G. raimondii. B: BAC clone 305A19 tagged by SSR marker NAU2015 (green) and chromosome D501 specific BAC clone tagged by SSR marker BNL3902 (red), G. raimondii. C: BAC clone 305A19 tagged by SSR marker NAU2015 (green) and chromosome Dh01, Ah01 specific BAC clones tagged by SSR marker BNL3902, BNL3580 (red), G. hirsutum. D: BAC clone 216B15 tagged by SSR marker NAU4891 (green) and chromosome Dh01, Ah01 specific BAC clones tagged by SSR marker BNL3902, BNL3580 (red), G. hirsutum.