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Figure 4 | Molecular Cytogenetics

Figure 4

From: Non-coding RNA derived from a conservative subtelomeric tandem repeat in chicken and Japanese quail somatic cells

Figure 4

PO41 repeat is transcribed in chicken skeletal muscle cells. FISH with PO41pos (green, upper row) and PO41neg (red, bottom row) probes on skeletal muscle cryosections. (a, a’) DNA/RNA hybridization revealed transcripts from both strands of PO41 repeat in cell nuclei. (b, b’) DNA/DNA hybridization (positive control) revealed clusters of PO41 repeat in all cell nuclei. (c, c’) RNase A treatment before DNA/RNA hybridization (negative control) removed all hybridization signals. Nuclei were counterstained with DAPI. Scale bars: 10 μm (a); 20 μm (b, c, a’); 30 μm (b’, c’).

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