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Figure 3 | Molecular Cytogenetics

Figure 3

From: Non-coding RNA derived from a conservative subtelomeric tandem repeat in chicken and Japanese quail somatic cells

Figure 3

Sensitivity of G-rich PO41 transcripts during the cell cycle to RNases with different substrate specificity. Treatment of MDCC-MSB1 cells with different RNases before or after (RNase H*) DNA/RNA FISH with PO41neg (red) probes. MDCC-MSB1 cells are shown at interphase, prophase, metaphase, anaphase and telophase stages. RNases used for treatments are indicated on the left. PO41 RNA was degraded by RiboShredder RNases cocktail, RNase A treatments and RNase H treatment performed after FISH. RNase H and RNase III treatments completely removed only dispersed G-rich transcripts at interphase and decreased the intensity of RNA FISH signals at other stages of cell cycle. DNA was counterstained with DAPI. Scale bar: 5 μm.

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Molecular Cytogenetics

ISSN: 1755-8166