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Figure 1 | Molecular Cytogenetics

Figure 1

From: Non-coding RNA derived from a conservative subtelomeric tandem repeat in chicken and Japanese quail somatic cells

Figure 1

PO41 tandem repeat is transcribed in chicken lymphoblastoid MDCC-MSB1 cells. FISH with PO41pos (green) and PO41neg (red) probes on chicken MDCC-MSB1 cells. (a, a’) DNA/RNA hybridization revealed transcripts from both strands of PO41 repeat in interphase nuclei. (b, b’) DNA/DNA hybridization (positive control) revealed clusters of PO41 repeat in interphase nuclei. (c, c’) RiboShredder RNase cocktail treatment before DNA/RNA hybridization (negative control) removed all hybridization signals. (d) 3D reconstruction of interphase nucleus with isosurfaces around G-rich transcripts of PO41 repeat (red) and chromatin (blue). DNA was counterstained with DAPI. Scale bars: 5 μm (a-c, a’-c’); 3 μm (d).

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Molecular Cytogenetics

ISSN: 1755-8166