Figure 4

Combined images of CC and FISH with single copy probes. A. Metaphase spread in GTG banding showing t(2;5;13) (p21;p15.1;q22). Arrows show abnormal chromosomes. B. The same metaphase as 4A with N-MYC (2p24) probe in red (control gene – LAF (2q11) – green). One of N-MYC signals is present on der(5). Arrows show abnormal chromosomes 2 and 5. C. The same metaphase as 4A and 4B, with cri-du-chat critical region probe – CTNND2 (5p15.2) – red. Control region, 5q13 – green. One of the CTNND2 signals is visible on der(13). Arrows show abnormal chromosomes 5 and 13. D. The same metaphase as 4A, 4B and 4C, with DLEU1 probe (13q14.3) – red. Control region, 13qter, is green. Both signals are present on normal 13 chromosome. The second DLEU1 signal is present on der(13), and the second control signal on der(2). Arrows show abnormal chromosomes 2 and 13.