Figure 7

Karyotype array of the clonal neoplasia RMT-CN 58, which evolved from a non-clonal rat mammary hyperplasia. The karyotype array of the neoplastic clone RMT-CN 58 was prepared from a three-dimensionally growing focal colony that had evolved from a culture of the non-clonal aneuploid mammary hyperplasia RMT 58 within several months in culture (Table 3). The karyotype array and the attached table show that the numbers of RMT-CN 58 chromosomes per cell were quasi-clonal, averaging 74 ± 3. The data also show that the copy numbers of the normal and of four clone-specific marker chromosomes of RMT-CN 58 were 55-100%-clonal. The individuality of the RMT-CN 58 karyotype is again evident from comparisons with the karyotype arrays of the carcinomas RMT 47–3 and 37–2 and the neoplastic clone RMT-CN 65 shown in Figures 3, 5 and 6. The RMT-CN 58 clone stands out by a high level of ongoing karyotypic variations, generating karyotypes with up to five new non-clonal aneusomies per cell.