Figure 2

The der(8) and the der(22) junction fragments of the t(8;22). A. Diagram for the translocation-specific PCR system. Chromosome 8 is indicated in white, while chromosome 22 is depicted in grey. Hatched boxes indicate PATRR arms. Translocation-specific PCR was performed with one primer designed at the flanking region of PATRR8 (grey primers) and with the other primer at the flanking region of PATRR22 (black primers). B. Results of family 1. Upper panel indicates results for the der(8), while lower panel indicates those of the der(22). Lane M, size markers; lane 1, FHU13-031 (proband); lane 2, FHU13-032 (father); lane 3, FHU13-033 (mother); lanes 4 and 5, normal healthy controls; lane 6, water control; lanes 7 and 8, balanced t(8;22) translocation carriers unrelated to the family. C. Results of family 2. Lane M, size markers; lane 1, FHU13-027 (proband); lanes 2 and 3, normal healthy controls; lane 4, water control; lanes 5 and 6, balanced t(8;22) translocation carriers unrelated to the family.