Table 1 Primers used for qPCR, qRT-PCR, and RT-PCR
Primer name | Gene name (GenBank accession number) | exon number | Primer sequence (5′ → 3′) | *Tm (°C) |
|---|---|---|---|---|
qPCR | Â | Â | Â | Â |
primer pair #1F | TCF7L1 | exon1 | F: ACGAGCTGATCCCCTTCC | 60.16 |
primer pair #1R | (NM_031283) | R: CTGCTCTGGTTCTCCGACTC | 60.14 | |
primer pair #2F | TCF7L1 | exon12 | F: GCAAGAAGCCATGTGTTCAG | 59.45 |
primer pair #2R | (NM_031283) | R: GGTTTCTGGTTTGGTGGTGA | 60.79 | |
primer pair #3F | TMEM150A | exon8 | F: TACGAGTTTGGGGCAGTCTC | 60.25 |
primer pair #3R | (NM_001031738) | R: CCTCCCCAGACCTTAGATCA | 59.79 | |
GAPDH-F | GAPDH | exon6 | F: GATCATCAGCAATGCCTCCT | 60.4 |
GAPDH-R | (NM_001289745) | R: ATGGCATGGACTGTGGTCAT | 60.4 | |
qRT-RCR | Â | Â | Â | Â |
primer pair #1F | TCF7L1 | exon1 | F: ACGAGCTGATCCCCTTCC | 60.16 |
primer pair #1R | (NM_031283) | R: CTGCTCTGGTTCTCCGACTC | 60.14 | |
primer pair #2F | TCF7L1 | exon12 | F: GCAAGAAGCCATGTGTTCAG | 59.45 |
primer pair #2R | (NM_031283) | R: GGTTTCTGGTTTGGTGGTGA | 60.79 | |
primer pair #4F | USP39 | exon5 | F: ATGTTCCTCCTCTCCGGAAC | 60.46 |
primer pair #4R | (NM_006590) | R: AGCATCTCATGGGGAGACAC | 60.08 | |
primer pair #5F | USP39 | exon10 | F: GACCTCATTGCCAACATCGT | 60.23 |
primer pair #5R | (NM_006590) | exon11 | R: GTGTGATCATCTGGGGAAGG | 60.33 |
CAPG-1F | CAPG | exon3 | F: CTCCATTCCCAGGCTCAGT | 60.21 |
CAPG-1R | (NM_001747) | R: GAAACCTCTTCTGGGCCATT | 60.44 | |
CAPG-2F | CAPG | exon9 | F: CGAAAAGCGAATGAGAAGGA | 60.46 |
CAPG-2R | (NM_001747) | exon10 | R: CCACCCTCATTTCCAGTCC | 60.31 |
VAMP8-F | VAMP8 | exon 3 | F: GAGGAAGCCAGTGAAGGTGG | 60.6 |
VAMP8-R | (NM_003761) | R: CAGATCCTCTGTCTTGTTGCG | 59.44 | |
RNF181-F | RNF181 | exon1 | F: ACCAACATGCTGCTGGAGC | 60 |
RNF181-R | (NM_016494) | R: TCTCAACCACAGTCTTGGCAG | 59 | |
GAPDH-F | GAPDH | exon6 | F: GATCATCAGCAATGCCTCCT | 60.4 |
GAPDH-R | (NM_001289745) | R: ATGGCATGGACTGTGGTCAT | 60.4 | |
RT-PCR | Â | Â | Â | Â |
Fusion-USP39-F | USP39 | exon6 | F: ATGTTCCTCCTCTCCGGAAC | 60.46 |
Fusion-TCF7L1-R | TCF7L1 (USP39/TCF7L1) | exon5 | R: TGTCTTTGGATCGATCTCTGG | 60.20 |