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Table 1 Detection of the derivative chromosomes in single S- and G0/G1 cells of three cell lines

From: Single cell segmental aneuploidy detection is compromised by S phase

A
  dup(7p) 25.16 Mb (40 probes)   dup(9p) 24 Mb (42 probes) del(18p) 8 Mb (28 probes)   dup(18p) 9.3 Mb (30 probes) del(20p)a1.7 Mb (8 probes)
S_1_1 25 S_2_1 26 3a S_3_1 7 7
S_1_2 11 S_2_2 21 13 S_3_2 14 7
S_1_3 13 S_2_3 19 21 S_3_3 4a 6
S_1_4 18 S_2_4 29 20 S_3_4 16 7
S_1_5 8a S_2_5 22 25 S_3_5 11 6
S_1_6 9a S_2_6 25 18 S_3_6 13 8
S_1_7 18 S_2_7 28 21    
S_1_8 13 S_2_8 27 22    
S_1_9 10a S_2_9 18 24    
S_1_10 27 S_2_10 31 22    
G0/G1_1_1 23 G0/G1_2_1 16 13 G0/G1_3_1 2a 7
G0/G1_1_2 20 G0/G1_2_2 19 17 G0/G1_3_2 17 6
G0/G1_1_3 18 G0/G1_2_3 25 19 G0/G1_3_3 15 7
G0/G1_1_4 28 G0/G1_2_4 36 23 G0/G1_3_4 19 5
G0/G1_1_5 24 G0/G1_2_5 31 17 G0/G1_3_5 17 6
G0/G1_1_6 20 G0/G1_2_6 23 20 G0/G1_3_6 17 7
G0/G1_1_7 25 G0/G1_2_7 22 20 G0/G1_3_7 23 4
   G0/G1_2_8 31 19    
B        
  Underestimation b No call a Total number of false negative Total number of false positive Total number of cells
S 14 3 17 5c 26
G0/G1 3 1 4 0 22
  1. (A) Number of probes indicating the presence of an imbalance in the regions of interest according to the BAC array log2 intensity ratios of single cells per cell phase and cell line. In brackets the number of probes in the regions of interest. The number of probes indicating the presence of the imbalance of interest was significantly higher in G0/G1- compared to S-phase cells (Mann Whitney p = 0.029). (B) Total number of miss-calls per cell cycle phase. The accurate imbalance detection rate is significantly higher in G0/G1- compared to S-phase cells (Chi-square test p = 0.001).
  2. aThe imbalance was not automatically indicated by the software.
  3. bThe proximal or distal end of the imbalance was very weakly detected or not detected at all.
  4. cThe log2 intensity ratios for 1 cell were indicative of a deletion in the control sample, while there is a duplication in the same region in the cell line of interest.