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Table 1 Juxtaposition of conventional CGH- and SNP-array techniques to the combined approach chosen in this study

From: Diagnostic utility of novel combined arrays for genome-wide simultaneous detection of aneuploidy and uniparental isodisomy in losses of pregnancy

  Conventional aCGH Conventional SNP-array Novel CGH + SNP-array
Array design One target consists of one 60mer oligonucleotide Allele-specific 60mer olionucleotide targets, arranged in quartets differing in a single base each are arranged for SNP-detection CGH + SNP-arrays contain both kinds of probes
Type of aberrations targeted Copy number aberrations Copy number and copy number neutral deviations (LOH) Copy number and copy number neutral deviations (LOH)
Mode of hybridization Comparative hybridization with a reference sample Sample hybridization only Comparative hybridization with a reference sample
Mode of detection/ algorithm settings Copy number determination by comparison with a diploid reference sample Determination of heterozygosity status by measuring of A- and B-alleles and in silico comparison to reference sample sets, deduction of copy number status from that Combined detection: Only one allele is measured, the total number of alleles at given loci are estimated from CGH-probe signals