Expression analysis. a) MSI2 and b) HOXA9 are over-expressed in the present patient with NUP98/HOXA13. BC1 and BC2: two other cases of Ph + blast crisis CML with additional karyotypic aberrations over-expressing MSI2 but not HOXA9. Expression values were referred to the average of two references. Fluorescence data were analyzed with the Second Derivative Maximum method; gene expression was expressed as Cp (Crossing point) values. c) Significance for MSI2 expression was tested by Mann–Whitney test (*p < 0.05/3); values are expressed as means +/− SD; AA. Additional aberrations.