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Figure 1 | Molecular Cytogenetics

Figure 1

From: PAX5 fusion genes in t(7;9)(q11.2;p13) leukemia: a case report and review of the literature

Figure 1

Cytogenetic and molecular genetic analysis of a PAX5-ELN positive case. (A) Karyogram; red arrow indicates the derivative chromosome der(9)t(7;9)(q11.23;p13) (refined karyotype using molecular methods). (B) FISH using PAX5-specific BAC clones showing a 3′-end deletion: 5′-end-specific clone (red signals); 3′-end-specific clone (green signals); black and red arrows indicate the normal and derivative chromosome, respectively. (C) FISH using PAX5- and ELN-specific BAC clones showing a co-localization: PAX5 5′-end-specific clone (red signals); ELN 3′-end-specific clone (green signals); arrows indicate the normal chromosomes 9 and 12 (white) and the derivative chromosome (black). (D) RT-PCR using primers located in PAX5 exon 2–3 and ELN exon 6 resulting in amplification of PAX5-ELN fusion transcripts. M, molecular weight marker DNA-mix ladder (Peqlab); lane 1, patient No. 5; lane 2, normal control. (E) Sequence chromatogram of the PAX5-ELN fusion junction showing the fusion between exon 7 of PAX5 and exon 5 of ELN.

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