FISH mapping of two microsatellite motifs in male and female of A. parapulchella . FISH mapping of (AGAT)8 in male (A) and female (B), and (AC)15 in male (C) and female (D). Arrowheads indicate hybridization signals on the Y chromosome (A, C). Arrows indicate hybridization signals on two pair of autosomes (C, D). Scale bars = 10 μm.