Skip to main content
Figure 4 | Molecular Cytogenetics

Figure 4

From: Atypical rearrangement involving 3′-IGH@ and a breakpoint at least 400 Kb upstream of an intact MYC in a CLL patient with an apparently balanced t(8;14)(q24.1;q32) and negative MYC expression

Figure 4

Further Examination of the Rearrangement Involving the MYC and IGH@ Loci using Sequential Metaphase FISH in our Patient’s Peripheral Blood. A: G-Banding metaphase spread showing an apparently balanced t(8;14)(q24.1;q32). B: The IGH@ break apart probe reveals splitting of signals (1Y1G1R) indicative of rearrangement, with 5’-IGH@ relocated to der 8q and 3’-IGH@ retained on der 14q. C: The IGH-MYC fusion probe shows 1Y2G1R2A, fusion of MYC-IGH der 14q32 (yellow), and deletion of MYC on der 8q24.1 (green, no red). It also shows normal signal patterns for the other chromosome 8 (aqua for centromere and red for MYC), and chromosome 14 (green). D: The MYC break apart probe detects 2Y signals, one on normal chromosome 8 and the other is removed from der 8q24.1 and relocated to der 14q32. These findings suggest that there is neither splitting of signals, or a deletion in between the red and green signals.

Back to article page

Molecular Cytogenetics

ISSN: 1755-8166