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Figure 13 | Molecular Cytogenetics

Figure 13

From: Individual karyotypes at the origins of cervical carcinomas

Figure 13

Karyotype arrays of two morphological variants and a methotrexate-resistant variant of the cervical carcinoma HeLa. The table of the chromosomes and the arrays of HeLa variants show that the total chromosome number of the morphological variant R (round) is 71±3, that of the morphological variant S (fusiform) is 67±2, and that of the methotrexate-resistant clone of S is 66±6. These chromosome numbers are thus all quasi-clonal. The copy numbers of 55-100% of chromosomes were also clonal. Non-clonal chromosomes included intact chromosomes with near-clonal copy numbers and new non-clonal marker chromosomes, which were found in two and four of the established morphological HeLa variants and in nine of 20 cells of the new methotrexate-resistant HeLa S clone. Comparisons of the karyotype arrays of the three HeLa variants show that all three variants are phylogenetically closely related subspecies with the following distinctions: The morphological variant R differed from S in five copy numbers of intact chromosomes and in 7 R-specific and 8 S-specific marker chromosomes. The methotrexate-resistant S clone differed from the native S variant in nine copy numbers of intact chromosomes and in the loss of seven 30-90% clonal S markers. Comparison of the HeLa arrays with the arrays of the seven distinct carcinomas shown in Figures 6, 7, 8, 9, 10, 11 and 12 reveals again at a glance that all eight carcinomas tested so far have individual and clonal karyotypes.

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