Figure 1
From: On the significance of germline cytogenetic rearrangements at MYCN locus in neuroblastoma
Evaluation of MYCN status in the NB tumor of a patient with constitutional 2p duplication. A. Interphase FISH analysis of the MYCN status in the tumor “touch imprints” (red – MYCN, green – LAF at chromosome 2q11.2). B. array-CGH analysis of the MYCN status. DNA extracted from the tumor hybridized against constitutional DNA of the same patient (I) and against a pool of control reference DNA (II). X-axis: the log2 ratio, Y-axis: chromosomal coordinates according to NCBI36/hg18. C. qPCR analysis of the MYCN status. The dosage of the MYCN gene (three independent qPCR amplicons) and the control gene ZNF2 (located at chromosome 2q11.1).