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Table 1 The signal strength of the telomere and centromere probes after an 18-hour hybridization period at varying temperatures and conditions were rated as absent, poor, fair or strong

From: Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization

Probes Goat Serum Formaldehyde FISH
4°C RT 37°C 4°C RT 37°C
TelC Strong Strong Strong Strong Strong Strong Strong
TelG Poor HB Fair Strong Poor HB Fair Strong Strong
CENPB Box Absent Poor Strong Fair Strong Strong Strong
Cent Absent Absent Absent Absent Absent Absent Strong
  1. High background was indicated as, HB, frequently found accompanying that signal only at the specified conditions. FISH indicates the traditional FISH protocol was used.