Figure 3
From: Characterization of a case of follicular lymphoma transformed into B-lymphoblastic leukemia
Molecular delineation of complex rearrangement of the marker chromosome bearing MYC , IGH and BCL2 . (A) OncoChip™ | B-ALL Panel 2 and standard aCGH are shown for comparison with results indicative of two rearrangements (black arrows on top), one corresponding to the Constant region (class switch) and the other corresponding to the Joining region of the IGH locus as indicated. Locations of primers designed for subsequent confirmatory PCR reactions (red arrows), and the location and directionality of the MYC and BCL2 genes are also indicated. The lines originated from MYC and BCL2 indicate that MYC is inserted into the IGH, while IGH/BCL2 fusion is resulted from a translocation. (B) PCR reactions using the indicated primers confirm the insertion of MYC gene into IGH Constant region and translocation of BCL2 gene to the IGH Joining site.