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Figure 2 | Molecular Cytogenetics

Figure 2

From: Incidence and patterns of ALK FISH abnormalities seen in a large unselected series of lung carcinomas

Figure 2

Characterization of novel EML4-ALK transcription variants in a patient with lung adenocarcinoma. This patient had a typical positive break-apart FISH signal pattern of the ALK gene. A) Two transcription variants were detected, derived from a fusion of EML4 exon 17 to the 3 end portion of ALK exon 20, with 46 base pairs deleted from the 5 end of ALK exon 20. These two fusion transcripts were from the same gene rearrangement with either a 23-nucleotide or a 6-nucleotide insertion from EML4 intron 17. B) Putative proteins from EML4-ALK fusion transcripts. The transcript with an insertion of 23 nucleotides produced a truncated EML4 protein and no EML4-ALK fusion protein, as a result of an early stop codon. However, the transcript with an insertion of 6 nucleotides produced an EML4-ALK fusion protein, containing the N-terminal region of EML4 and the C-terminal region of ALK with the protein tyrosine kinase domain (PTKc).

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