Figure 1
Visualization of GFP-TRF1 and PNA FISH foci in Syrian hamster interphase nuclei. Confocal images were captured using the Leica TCS SP5 system. Maximal projections of series of confocal sections of the nuclei are shown (A and B). The nuclear DNA is counterstained with DAPI (blue). (A) The nucleus expressing GFP-TRF1. GFP-TRF1 foci (green) mark location of the blocks of telomeric repeats. The foci that form clusters are indicated by arrows: 1 - the cluster of two large foci; 2 – the cluster of two small foci. (B) The nucleus containing PNA FISH foci (green). The cluster of three small foci is indicated by the arrow. Rotation of 3D images of the nuclei 22o around x-axis using 3D projection tool of LAS AF software confirmed the association of foci in clusters. Magnified images of clusters (without rotation and rotated around x-axis) are shown on the right. Bars in A and B 10μm.