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Figure 1 | Molecular Cytogenetics

Figure 1

From: Cytomolecular characterization of de novo formed rye B chromosome variants

Figure 1

Characterization of mitotic rye ( Secale cereale ) B chromosome variants by FISH with the B-specific probes D1100 and CL11, the microsatellite (CAA) 10 , as well as with the Arabidopsis -type telomere repeat. (a) Standard Bs and its structural variants found in the rye line 7415. The B variants are classified into three different subtypes. Bar equals 10 μm. (b) Plant #11 carrying a long arm iso B, (c) Plant #4 carrying a small deficient B lacking the long arm, (d) Plant #6 carrying four standard Bs and one small B variant (arrow). (e) Standard B showing telomeric signals on both arms and a pericentromeric CL11 signal, (f) B-variant almost entirely labeled with CL11 sequence but with telomeric signals on one chromosome arm only, (g) B variant showing a high accumulation of CL11 signals and telomeric signals only on the short arm, (h) two small B variants, (i) metaphase of a wheat plant with rye short arm iso B showing telomeric signals on both arms of a small iso B. Inserts highlight B variants. Bar equals 20 μm.

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