FISH analysis using selective probes confirmed the aCGH results. (A) It showed only one signal of the LSI EGR1 (red) and two signals of LSI D5S21 (green), indicating a loss of the EGR1 gene. (B) One signal of the LSI c-MYC (yellow) demonstrates a loss of the MYC gene. (C) One signal of the LSI IGH (green) indicates a loss of the IGH gene, and the white circle indicates amplification of the FGFR3 gene (red). (D) One signal of the LSI TP53 indicates a loss of the TP53 gene.