24-color mFISH karyotyping (24XCyte).
A. A representative 24-color mFISH karyogram showing the unbalanced der(5)t(4;5) (arrow) as the sole cytogenetic abnormality. B. Single color gallery tool of normal chromosomes 4 and 5 and the unbalanced der(5)t(4;5). With this tool it is possible to show assigned false colors (FC) representing individual color schemes of labeled chromosomes arranged in its capture sequence (fluorescein isothiocyanate) FITC, (spectrum orange) SpO, (texas red) TR, (cyanine 5) Cy5, (7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester) DEAC, together with an inverted grayscale image of the (4',6-diamidino-2-phenylindole) DAPI image (Inv). The top panel shows a normal chromosome 4 (#4) labeled with FITC, the middle panel shows a normal chromosome 5 (#5) labeled with SpO, and the bottom panel shows the unbalanced der(5)t(4;5) harboring labels from both chromosome 4 and 5. With the inverted gray scale image of der(5) it is not possible to identify the translocation.