Nuclei with and without sSMC(16) derived from lymphocytes. A) Representative interphase nucleus for case 3. In the figure the position of the sSMC is highlighted (arrowhead) and the pseudocolors together with the applied probes are given. Chromosomes 19 are in close together and intermediate to peripheral, and both chromosomes 16 and the sSMC are intermediate to peripheral positioned. The sSMC is not colocalized with any of the tested chromosomes. B) Representative interphase nucleus of case 6 using probe set for case 3. One chromosome 19 is in central position and appears as two separate spots due to image processing (arrowheads) and the other chromosome 19 is in intermediate to peripheral position. One chromosome 16 is in central to intermediate, the other in peripheral to intermediate position. N.B. Animations of the corresponding nuclei are available as additional file - film 3.